Cross-talk and regulatory interactions between the essential response regulator RpaB and cyanobacterial circadian clock output

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Title: Cross-talk and regulatory interactions between the essential response regulator RpaB and cyanobacterial circadian clock output
Authors: Espinosa Manzano, Javier | Boyd, Joseph S. | Cantos, Raquel | Salinas, Paloma | Golden, Susan S. | Contreras, Asunción
Research Group/s: Transducción de Señales en Bacterias
Center, Department or Service: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología
Keywords: Signaling network | Transcription regulation | Chronobiology
Knowledge Area: Genética
Issue Date: 17-Feb-2015
Publisher: National Academy of Sciences
Citation: Proceedings of the National Academy of Sciences. 2015, 112(7): 2198-2203. doi:10.1073/pnas.1424632112
Abstract: The response regulator RpaB (regulator of phycobilisome associated B), part of an essential two-component system conserved in cyanobacteria that responds to multiple environmental signals, has recently been implicated in the control of cell dimensions and of circadian rhythms of gene expression in the model cyanobacterium Synechococcus elongatus PCC 7942. However, little is known of the molecular mechanisms that underlie RpaB functions. In this study we show that the regulation of phenotypes by RpaB is intimately connected with the activity of RpaA (regulator of phycobilisome associated A), the master regulator of circadian transcription patterns. RpaB affects RpaA activity both through control of gene expression, a function requiring an intact effector domain, and via altering RpaA phosphorylation, a function mediated through the N-terminal receiver domain of RpaB. Thus, both phosphorylation cross-talk and coregulation of target genes play a role in the genetic interactions between the RpaA and RpaB pathways. In addition, RpaB∼P levels appear critical for survival under light:dark cycles, conditions in which RpaB phosphorylation is environmentally driven independent of the circadian clock. We propose that the complex regulatory interactions between the essential and environmentally sensitive NblS-RpaB system and the SasA-RpaA clock output system integrate relevant extra- and intracellular signals to the circadian clock.
Sponsor: This work was supported by Spanish Ministry of Economy and Competitivity Grant BFU2012-33364 (to A.C.); Generalitat Valenciana Grants ACOMP/2014/144 (to A.C.) and GV/2014/073 (to J.E.); National Institutes of Health Grant R01GM062419 (to S.S.G.); and European Molecular Biology Organization postdoctoral Fellowship ASTF 74–2013 (to J.E.).
URI: http://hdl.handle.net/10045/58326
ISSN: 1091-6490
DOI: 10.1073/pnas.1424632112
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2015 National Academy of Sciences
Peer Review: si
Publisher version: http://dx.doi.org/10.1073/pnas.1424632112
Appears in Collections:INV - TSB - Artículos de Revistas

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