Tuning fresh: radiation through rewiring of central metabolism in streamlined bacteria

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Título: Tuning fresh: radiation through rewiring of central metabolism in streamlined bacteria
Autor/es: Eiler, Alexander | Mondav, Rhiannon | Sinclair, Lucas | Fernandez-Vidal, Leyden | Scofield, Douglas G. | Schwientek, Patrick | Martinez-Garcia, Manuel | Torrents, David | McMahon, Katherine D. | Andersson, Siv G.E. | Stepanauskas, Ramunas | Woyke, Tanja | Bertilsson, Stefan
Grupo/s de investigación o GITE: Ecología Microbiana Molecular
Centro, Departamento o Servicio: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología
Palabras clave: Tuning fresh | Radiation | Central metabolism | Streamlined bacteria
Área/s de conocimiento: Microbiología
Fecha de publicación: 19-ene-2016
Editor: Nature Publishing Group
Cita bibliográfica: The ISME Journal. 2016, 10: 1902-1914. doi:10.1038/ismej.2015.260
Resumen: Most free-living planktonic cells are streamlined and in spite of their limitations in functional flexibility, their vast populations have radiated into a wide range of aquatic habitats. Here we compared the metabolic potential of subgroups in the Alphaproteobacteria lineage SAR11 adapted to marine and freshwater habitats. Our results suggest that the successful leap from marine to freshwaters in SAR11 was accompanied by a loss of several carbon degradation pathways and a rewiring of the central metabolism. Examples for these are C1 and methylated compounds degradation pathways, the Entner–Doudouroff pathway, the glyoxylate shunt and anapleuretic carbon fixation being absent from the freshwater genomes. Evolutionary reconstructions further suggest that the metabolic modules making up these important freshwater metabolic traits were already present in the gene pool of ancestral marine SAR11 populations. The loss of the glyoxylate shunt had already occurred in the common ancestor of the freshwater subgroup and its closest marine relatives, suggesting that the adaptation to freshwater was a gradual process. Furthermore, our results indicate rapid evolution of TRAP transporters in the freshwater clade involved in the uptake of low molecular weight carboxylic acids. We propose that such gradual tuning of metabolic pathways and transporters toward locally available organic substrates is linked to the formation of subgroups within the SAR11 clade and that this process was critical for the freshwater clade to find and fix an adaptive phenotype.
Patrocinador/es: This work was supported by the Swedish Research Council (Grant Numbers 2012-4592 to AE and 2012-3892 to SB) and the Communiy Sequencing Programme of the US Department of Energy Joint Genome Institute. The work conducted by the US Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported under Contract No. DE-AC02-05CH11231.
URI: http://hdl.handle.net/10045/57595
ISSN: 1751-7362 (Print) | 1751-7370 (Online)
DOI: 10.1038/ismej.2015.260
Idioma: eng
Tipo: info:eu-repo/semantics/article
Derechos: This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/
Revisión científica: si
Versión del editor: http://dx.doi.org/10.1038/ismej.2015.260
Aparece en las colecciones:INV - EMM - Artículos de Revistas

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