Correlation between SD-OCT, immunocytochemistry and functional findings in an animal model of retinal degeneration

Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10045/54650
Información del item - Informació de l'item - Item information
Título: Correlation between SD-OCT, immunocytochemistry and functional findings in an animal model of retinal degeneration
Autor/es: Cuenca, Nicolás | Fernández Sánchez, Laura | Sauvé, Yves | Segura, Francisco | Martínez Navarrete, Gema Concepción | Tamarit, José Manuel | Fuentes-Broto, Lorena | Sánchez Cano, Ana | Pinilla Lozano, Isabel
Grupo/s de investigación o GITE: Neurobiología del Sistema Visual y Terapia de Enfermedades Neurodegenerativas (NEUROVIS)
Centro, Departamento o Servicio: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología
Palabras clave: Retinal degeneration | Retinitis pigmentosa | P23H rat | Visual acuity | Electroretinogram | Optical coherence tomography | Autofluorescence | Immunohistochemistry
Área/s de conocimiento: Biología Celular
Fecha de publicación: 22-dic-2014
Editor: Frontiers Media
Cita bibliográfica: Cuenca N, Fernández-Sánchez L, Sauvé Y, Segura FJ, Martínez-Navarrete G, Tamarit JM, Fuentes-Broto L, Sanchez-Cano A and Pinilla I (2014) Correlation between SD-OCT, immunocytochemistry and functional findings in an animal model of retinal degeneration. Front. Neuroanat. 8:151. doi: 10.3389/fnana.2014.00151
Resumen: Purpose: The P23H rhodopsin mutation is an autosomal dominant cause of retinitis pigmentosa (RP). The degeneration can be tracked using different anatomical and functional methods. In our case, we evaluated the anatomical changes using Spectral-Domain Optical Coherence Tomography (SD-OCT) and correlated the findings with retinal thickness values determined by immunocytochemistry.Methods: Pigmented rats heterozygous for the P23H mutation, with ages between P18 and P180 were studied. Function was assessed by means of optomotor testing and ERGs. Retinal thicknesses measurements, autofluorescence and fluorescein angiography were performed using Spectralis OCT. Retinas were studied by means of immunohistochemistry. Results: Between P30 and P180, visual acuity decreased from 0.500 to 0.182 cycles per degree (cyc/deg) and contrast sensitivity decreased from 54.56 to 2.98 for a spatial frequency of 0.089 cyc/deg. Only cone-driven b-wave responses reached developmental maturity. Flicker fusions were also comparable at P29 (42 Hz). Double flash-isolated rod-driven responses were already affected at P29. Photopic responses revealed deterioration after P29.A reduction in retinal thicknesses and morphological modifications were seen in OCT sections. Statistically significant differences were found in all evaluated thicknesses. Autofluorescence was seen in P23H rats as sparse dots. Immunocytochemistry showed a progressive decrease in the outer nuclear layer (ONL), and morphological changes. Although anatomical thickness measures were significantly lower than OCT values, there was a very strong correlation between the values measured by both techniques.Conclusions: In pigmented P23H rats, a progressive deterioration occurs in both retinal function and anatomy. Anatomical changes can be effectively evaluated using SD-OCT and immunocytochemistry, with a good correlation between their values, thus making SD-OCT an important tool for research in retinal degeneration.
Patrocinador/es: Dr. Pinilla and Dr. Cuenca were supported by grants from the Spanish Ministry of Economy and Competitiveness-FEDER (BFU2012-36845), Instituto de Salud Carlos III (FIS PI13/01124, PS0901854, PI042399 and RETICS RD12/0034/0010), Fundación Gangoiti, ONCE (Organización Nacional de Ciegos Españoles) and FUNDALUCE. Dr. Yves Sauvé is a recipient of the Barbara Tuck/MacPhee Family Vision Research Award in Macular Degeneration.
URI: http://hdl.handle.net/10045/54650
ISSN: 1662-5129
DOI: 10.3389/fnana.2014.00151
Idioma: eng
Tipo: info:eu-repo/semantics/article
Derechos: © 2014 Cuenca, Fernández-Sánchez, Sauvé, Segura, Martínez-Navarrete, Tamarit, Fuentes-Broto, Sanchez-Cano and Pinilla. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Revisión científica: si
Versión del editor: http://dx.doi.org/10.3389/fnana.2014.00151
Aparece en las colecciones:INV - NEUROVIS - Artículos de Revistas

Archivos en este ítem:
Archivos en este ítem:
Archivo Descripción TamañoFormato 
Thumbnail2014_Cuenca_etal_FrontNeuroanat.pdf1,55 MBAdobe PDFAbrir Vista previa


Este ítem está licenciado bajo Licencia Creative Commons Creative Commons