From community approaches to single-cell genomics: the discovery of ubiquitous hyperhalophilic Bacteroidetes generalists

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Título: From community approaches to single-cell genomics: the discovery of ubiquitous hyperhalophilic Bacteroidetes generalists
Autor/es: Gomariz, María | Martinez-Garcia, Manuel | Santos Sánchez, Fernando | Rodriguez-Valera, Francisco | Capella-Gutiérrez, Salvador | Gabaldón, Toni | Rosselló Móra, Ramon | Meseguer, Inmaculada | Anton, Josefa
Grupo/s de investigación o GITE: Ecología Microbiana Molecular | Análisis de Datos y Modelización de Procesos en Biología y Geociencias
Centro, Departamento o Servicio: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología | Universidad de Alicante. Departamento de Matemática Aplicada
Palabras clave: Community approaches | Single-cell | Genomics | Hyperhalophilic Bacteroidetes generalists
Área/s de conocimiento: Microbiología | Matemática Aplicada
Fecha de publicación: 2015
Editor: Nature Publishing Group
Cita bibliográfica: The ISME Journal. 2015, 9: 16–31. doi:10.1038/ismej.2014.95
Resumen: The microbiota of multi-pond solar salterns around the world has been analyzed using a variety of culture-dependent and molecular techniques. However, studies addressing the dynamic nature of these systems are very scarce. Here we have characterized the temporal variation during 1 year of the microbiota of five ponds with increasing salinity (from 18% to >40%), by means of CARD-FISH and DGGE. Microbial community structure was statistically correlated with several environmental parameters, including ionic composition and meteorological factors, indicating that the microbial community was dynamic as specific phylotypes appeared only at certain times of the year. In addition to total salinity, microbial composition was strongly influenced by temperature and specific ionic composition. Remarkably, DGGE analyses unveiled the presence of most phylotypes previously detected in hypersaline systems using metagenomics and other molecular techniques, such as the very abundant Haloquadratum and Salinibacter representatives or the recently described low GC Actinobacteria and Nanohaloarchaeota. In addition, an uncultured group of Bacteroidetes was present along the whole range of salinity. Database searches indicated a previously unrecognized widespread distribution of this phylotype. Single-cell genome analysis of five members of this group suggested a set of metabolic characteristics that could provide competitive advantages in hypersaline environments, such as polymer degradation capabilities, the presence of retinal-binding light-activated proton pumps and arsenate reduction potential. In addition, the fairly high metagenomic fragment recruitment obtained for these single cells in both the intermediate and hypersaline ponds further confirm the DGGE data and point to the generalist lifestyle of this new Bacteroidetes group.
Patrocinador/es: This work was supported by the projects CGL2012-39627-C03-01 and 02 of the Spanish Ministry of Economy and Competitiveness, which were also co-financed with FEDER support from the European Union. TG group research is funded in part by a grant from the Spanish Ministry of Economy and Competitiveness (BIO2012-37161), a grant from the Qatar National Research Fund grant (NPRP 5-298-3-086) and a grant from the European Research Council under the European Union’s Seventh Framework Programme (FP/2007-2013)/ERC (grant agreement no. ERC-2012-StG-310325).
ISSN: 1751-7362 (Print) | 1751-7370 (Online)
DOI: 10.1038/ismej.2014.95
Idioma: eng
Tipo: info:eu-repo/semantics/article
Derechos: © 2015 International Society for Microbial Ecology
Revisión científica: si
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