Characterisation of chlorate reduction in the haloarchaeon Haloferax mediterranei

Please use this identifier to cite or link to this item:
Información del item - Informació de l'item - Item information
Title: Characterisation of chlorate reduction in the haloarchaeon Haloferax mediterranei
Authors: Martínez-Espinosa, Rosa María | Richardson, David J. | Bonete, María-José
Research Group/s: Biotecnología de Extremófilos (BIOTECEXTREM)
Center, Department or Service: Universidad de Alicante. Departamento de Agroquímica y Bioquímica
Keywords: Halophile | Archaeon | Respiratory nitrate reductase | Chlorate reduction | Anaerobic respiration | Chlorite dismutase
Knowledge Area: Bioquímica y Biología Molecular
Issue Date: Apr-2015
Publisher: Elsevier
Citation: Biochimica et Biophysica Acta (BBA) - General Subjects. 2015, 1850(4): 587-594. doi:10.1016/j.bbagen.2014.12.011
Abstract: Background: Haloferax mediterranei is a denitrifying haloarchaeon using nitrate as a respiratory electron acceptor under anaerobic conditions in a reaction catalysed by pNarGH. Other ions such as bromate, perchlorate and chlorate can also be reduced. Methods: Hfx. mediterranei cells were grown anaerobically with nitrate as electron acceptor and chlorate reductase activity measured in whole cells and purified nitrate reductase. Results: No genes encoding (per)chlorate reductases have been detected either in the Hfx. mediterranei genome or in other haloarchaea. However, a gene encoding a chlorite dismutase that is predicted to be exported across the cytoplasmic membrane has been identified in Hfx. mediterranei genome. Cells did not grow anaerobically in presence of chlorate as the unique electron acceptor. However, cells anaerobically grown with nitrate and then transferred to chlorate-containing growth medium can grow a few generations. Chlorate reduction by the whole cells, as well as by pure pNarGH, has been characterised. No clear chlorite dismutase activity could be detected. Conclusions: Hfx. mediterranei pNarGH has its active site on the outer-face of the cytoplasmic membrane and reacts with chlorate and perchlorate. Biochemical characterisation of this enzymatic activity suggests that Hfx. mediterranei or its pure pNarGH could be of great interest for waste water treatments or to better understand biological chlorate reduction in early Earth or Martian environments. General significance: Some archaea species reduce (per)chlorate. However, results here presented as well as those recently reported by Liebensteiner and co-workers [1] suggest that complete perchlorate reduction in archaea follows different rules in terms of biological reactions.
Sponsor: This work was funded by research grant from the MINECO Spain (CTM2013-43147-R), MEC Spain (BIO2008-00082), Generalitat Valenciana (GV/2011/038) and University of Alicante (GRE09-25).
ISSN: 0304-4165 (Print) | 1872-8006 (Online)
DOI: 10.1016/j.bbagen.2014.12.011
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2014 Elsevier B.V.
Peer Review: si
Publisher version:
Appears in Collections:INV - BIOTECEXTREM - Artículos de Revistas
INV - AppBiochem - Artículos de Revistas

Files in This Item:
Files in This Item:
File Description SizeFormat 
Thumbnail2015_Martinez_etal_BBAGen_final.pdfVersión final (acceso restringido)568,98 kBAdobe PDFOpen    Request a copy
Thumbnail2015_Martinez_etal_BBAGen_rev.pdfVersión revisada (acceso abierto)209,68 kBAdobe PDFOpen Preview

Items in RUA are protected by copyright, with all rights reserved, unless otherwise indicated.