Interactions between Closely Related Bacterial Strains Are Revealed by Deep Transcriptome Sequencing

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Título: Interactions between Closely Related Bacterial Strains Are Revealed by Deep Transcriptome Sequencing
Autor/es: González-Torres, Pedro | Pryszcz, Leszek P. | Santos Sánchez, Fernando | Martinez-Garcia, Manuel | Gabaldón, Toni | Anton, Josefa
Grupo/s de investigación o GITE: Ecología Microbiana Molecular
Centro, Departamento o Servicio: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología | Universidad de Alicante. Instituto Multidisciplinar para el Estudio del Medio "Ramón Margalef"
Palabras clave: Closely related bacterial strains | Deep transcriptome sequencing
Área/s de conocimiento: Microbiología
Fecha de publicación: dic-2015
Editor: American Society for Microbiology
Cita bibliográfica: González-Torres P, Pryszcz LP, Santos F, Martínez-García M, Gabaldón T, Antón J. 2015. Interactions between closely related bacterial strains are revealed by deep transcriptome sequencing. Appl Environ Microbiol 81:8445–8456. doi:10.1128/AEM.02690-15
Resumen: Comparative genomics, metagenomics, and single-cell technologies have shown that populations of microbial species encompass assemblages of closely related strains. This raises the question of whether individual bacterial lineages respond to the presence of their close relatives by modifying their gene expression or, instead, whether assemblages simply act as the arithmetic addition of their individual components. Here, we took advantage of transcriptome sequencing to address this question. For this, we analyzed the transcriptomes of two closely related strains of the extremely halophilic bacterium Salinibacter ruber grown axenically and in coculture. These organisms dominate bacterial assemblages in hypersaline environments worldwide. The strains used here cooccurred in the natural environment and are 100% identical in their 16S rRNA genes, and each strain harbors an accessory genome representing 10% of its complete genome. Overall, transcriptomic patterns from pure cultures were very similar for both strains. Expression was detected along practically the whole genome albeit with some genes at low levels. A subset of genes was very highly expressed in both strains, including genes coding for the light-driven proton pump xanthorhodopsin, genes involved in the stress response, and genes coding for transcriptional regulators. Expression differences between pure cultures affected mainly genes involved in environmental sensing. When the strains were grown in coculture, there was a modest but significant change in their individual transcription patterns compared to those in pure culture. Each strain sensed the presence of the other and responded in a specific manner, which points to fine intraspecific transcriptomic modulation.
Patrocinador/es: The group of J.A. is funded by grant CGL2012-39627-C03-01 from the Spanish Ministry of Economy and Competitiveness (MINECO), which is cofinanced with FEDER support from the European Union. P.G.-T. was an FPI-MINECO fellow. Research by the group of T.G. is funded in part by a grant from the Spanish Ministry of Economy and Competitiveness (BIO2012-37161), a grant from the Qatar National Research Fund (NPRP 5-298-3-086), and a grant from the European Research Council under the European Union’s Seventh Framework Programme (FP/2007-2013)/ERC (grant agreement no. ERC-2012-StG-310325). L.P.P. was funded through the La Caixa-CRG international fellowship program.
URI: http://hdl.handle.net/10045/51655
ISSN: 0099-2240 (Print) | 1098-5336 (Online)
DOI: 10.1128/AEM.02690-15
Idioma: eng
Tipo: info:eu-repo/semantics/article
Derechos: © 2015, American Society for Microbiology
Revisión científica: si
Versión del editor: http://dx.doi.org/10.1128/AEM.02690-15
Aparece en las colecciones:INV - EMM - Artículos de Revistas
Investigaciones financiadas por la UE

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