Unveiling viral–host interactions within the ‘microbial dark matter’

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Título: Unveiling viral–host interactions within the ‘microbial dark matter’
Autor/es: Martinez-Garcia, Manuel | Santos Sánchez, Fernando | Moreno-Paz, Mercedes | Parro, Víctor | Anton, Josefa
Grupo/s de investigación o GITE: Ecología Microbiana Molecular
Centro, Departamento o Servicio: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología
Palabras clave: Viruses | Hosts | Interactions | Microbial dark matter
Área/s de conocimiento: Microbiología
Fecha de publicación: 14-ago-2014
Editor: Macmillan Publishers
Cita bibliográfica: Nature Communications. 2014, 5: 4542. doi:10.1038/ncomms5542
Resumen: Viruses control natural microbial communities. Identification of virus–host pairs relies either on their cultivation or on metagenomics and tentative assignment based on genomic signatures. Both approaches have severe drawbacks when aiming to target such pairs within the uncultured majority. Here we present an unambiguous way to assign viruses to hosts that does not rely on any previous information about either of them nor requires their cultivation. First, genomic contents of individual cells present in an environmental sample are retrieved by means of single-cell genomic technologies. Then, individual cell genomes are hybridized against a set of individual viral genomes from the same sample, previously immobilized on a microarray. Infected cells will yield positive hybridization as they carry viral genomes, which can be then sequenced and characterized. Using this method, we pinpoint viruses infecting the ubiquitous hyperhalophilic Nanohaloarchaeota, included in the so-called ‘microbial dark matter’ (the uncultured fraction of the microbial world).
Patrocinador/es: This work was supported by the projects CGL2012-39627-C03-01 (to J.A.) and AYA2011-24803 (to V.P.) of the Spanish Ministry of Science and Innovation, which are co-financed with FEDER support from the European Union.
URI: http://hdl.handle.net/10045/47145
ISSN: 2041-1723
DOI: 10.1038/ncomms5542
Idioma: eng
Tipo: info:eu-repo/semantics/article
Derechos: © 2014 Macmillan Publishers Limited
Revisión científica: si
Versión del editor: http://dx.doi.org/10.1038/ncomms5542
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Thumbnail2014-Nat-Com-Assigning-viruses-to-hosts.pdfVersión final (acceso restringido)546,16 kBAdobe PDFAbrir    Solicitar una copia
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ThumbnailFig1.tifFigure 1. Experimental design used to assign viruses to hosts in natural assemblages1,15 MBTIFFAbrir
Fig2.tifFigure 2. Virus NHV-1 infecting nanohaloarchaeon host D141,86 MBTIFFAbrir
Fig3.tifFigure 3. Principal component analyses (PCAs) of the dinucleotide frequency signatures of viruses and hosts6,14 MBTIFFAbrir


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