Capacitation and acrosome reaction changes α-tubulin immunodistribution in human spermatozoa

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Title: Capacitation and acrosome reaction changes α-tubulin immunodistribution in human spermatozoa
Authors: Francou, María Manuela | Ten Morro, Jorge | Bernabeu, Rafael | Juan Herrero, Joaquín de
Research Group/s: Biotecnología
Center, Department or Service: Universidad de Alicante. Departamento de Biotecnología
Keywords: Acrosome reaction | Capacitation | Cytoskeleton | Immunocytochemistry | Spermatozoa | Tubulin
Knowledge Area: Biología Celular
Issue Date: Feb-2014
Publisher: Elsevier
Citation: Reproductive BioMedicine Online. 2014, 28(2): 246-250. doi:10.1016/j.rbmo.2013.10.001
Abstract: Tubulin is a protein constituent of cytoskeletal microtubules, closely related to sperm motility. However, the changes in tubulin distribution following capacitation and acrosome reaction are poorly understood. This study immunolocalized and quantified the expression of α-tubulin in fresh, capacitated and acrosome-reacted samples. Immunocytochemical data showed that in capacitated and acrosome-reacted spermatozoa, α-tubulin is labelled throughout most of the flagellum (⩾66.66%). However, the mean α-tubulin-labelled area in these samples was significantly lower than in fresh samples (P < 0.05). Thus, there are different sperm clusters distinguished by their α-tubulin immunoreactivity and this could be directly linked to structural changes following capacitation and acrosome reaction.
Sponsor: This project was supported by the Cátedra de Medicina de la Reproducción, Alicante University-Instituto Bernabeu, Grant 4–12I to JDJ, as well as the Vicerrectorado de Innovación, University of Alicante, Spain, Grant Vigrob-137 to JDJ.
ISSN: 1472-6483 (Print) | 1472-6491 (Online)
DOI: 10.1016/j.rbmo.2013.10.001
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2013, Reproductive Healthcare Ltd. Published by Elsevier Ltd.
Peer Review: si
Publisher version:
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