Diversity of extremely halophilic cultivable prokaryotes in Mediterranean, Atlantic and Pacific solar salterns: Evidence that unexplored sites constitute sources of cultivable novelty

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Title: Diversity of extremely halophilic cultivable prokaryotes in Mediterranean, Atlantic and Pacific solar salterns: Evidence that unexplored sites constitute sources of cultivable novelty
Authors: Viver, Tomeu | Cifuentes, Ana | Díaz, Sara | Rodríguez-Valdecantos, Gustavo | González, Bernardo | Anton, Josefa | Rosselló Móra, Ramon
Research Group/s: Ecología Microbiana Molecular
Center, Department or Service: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología
Keywords: Halophilic | MALDI-TOF MS | Large-scale cultivation | OTUs | OPUs | Salterns
Knowledge Area: Microbiología
Issue Date: Jun-2015
Publisher: Elsevier
Citation: Systematic and Applied Microbiology. 2015, 38(4): 266-275. doi:10.1016/j.syapm.2015.02.002
Abstract: The culturable fraction of aerobic, heterotrophic and extremely halophilic microbiota retrieved from sediment and brine samples of eight sampling sites in the Mediterranean, Canary Islands and Chile was studied by means of a tandem approach combining large-scale cultivation, MALDI-TOF MS targeting whole cell biomass, and phylogenetic reconstruction based on 16S rRNA gene analysis. The approach allowed the identification of more than 4200 strains and a comparison between different sampling sites. The results indicated that the method constituted an excellent tool for the discovery of taxonomic novelty. Four new genera and nine new species could be identified within the archaeal family Halobacteriaceae, as well as one new bacterial species, and a representative of Salinibacter ruber phylotype II, a group that had been refractory to isolation for the last fifteen years. Altogether, the results indicated that in order to provide better yields for the retrieval of novel taxa from the environment, performance of non-redundant environment sampling is recommended together with the screening of large sets of strains.
Sponsor: The current study was funded with the scientific support given by the Spanish Ministry of Economy through the projectsCGL2012-39627-C03-01 and CGL2012-39627-C03-03, which were also supported with European Regional Development Fund (FEDER) funds, and the preparatory phase of the Microbial Resource Research Infrastructure (MIRRI) funded by the EU (grant number 312251). In addition, the funding from competitive research groups (Microbiology) of the Government of the Balearic Islands (also co-supported with FEDER funds), is also acknowledged. TVP acknowledges the predoctoral fellowship of the Ministerio de Economía y Competitividad of the Spanish Government for the FPI fellowship (Nr BES-2013-064420) supporting his research activities.
URI: http://hdl.handle.net/10045/46890
ISSN: 0723-2020 (Print) | 1618-0984 (Internet)
DOI: 10.1016/j.syapm.2015.02.002
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2015 Elsevier GmbH.
Peer Review: si
Publisher version: http://dx.doi.org/10.1016/j.syapm.2015.02.002
Appears in Collections:INV - EMM - Artículos de Revistas

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