Eukarya associated with the stony coral Oculina patagonica from the Mediterranean Sea

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Título: Eukarya associated with the stony coral Oculina patagonica from the Mediterranean Sea
Autor/es: Rubio-Portillo, Esther | Souza-Egipsy, Virginia | Ascaso Ciria, Carmen | Ríos Murillo, Asunción de los | Ramos-Esplá, Alfonso A. | Anton, Josefa
Grupo/s de investigación o GITE: Recursos Hídricos y Desarrollo Sostenible | Biología Marina | Ecología Microbiana Molecular
Centro, Departamento o Servicio: Universidad de Alicante. Departamento de Ciencias del Mar y Biología Aplicada | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología
Palabras clave: Oculina patagonica | Symbiodinium | DGGE | ITS | 18S rRNA | 16S rRNA
Área/s de conocimiento: Zoología | Microbiología
Fecha de publicación: oct-2014
Editor: Elsevier
Cita bibliográfica: Marine Genomics. 2014, 17: 17-23. doi:10.1016/j.margen.2014.06.002
Resumen: Oculina patagonica is a putative alien scleractinian coral from the Southwest Atlantic that inhabits across the Mediterranean Sea. Here, we have addressed the diversity of Eukarya associated with this coral and its changes related to the environmental conditions and coral status. A total of 46 colonies of O. patagonica were taken from Alicante coast (Spain) and Pietra Ligure coast (Italy) and analyzed using denaturing gradient gel electrophoresis (DGGE) of the small-subunit 18S rRNA and 16S plastid rRNA genes, internal transcribed spacer region 2 (ITS 2) analyses, and electron microscopy. Our results show that Eukarya and plastid community associated to O. patagonica change with environmental conditions and coral status. Cryptic species, which can be difficult to identify by optical methods, were distinguished by 18S rRNA gene DGGE: the barnacle Megatrema anglicum, which was detected at two locations, and two boring sponges related to Cliona sp. and Siphonodictyon coralliphagum detected in samples from Tabarca and Alicante Harbour, respectively. Eukaryotic phototrophic community from the skeletal matrix of healthy corals was dominated by Ochrosphaera sp. while bleached corals from the Harbour and Tabarca were associated to different uncultured phototrophic organism. Differences in ultrastructural morphologies of the zooxanthellae between healthy and bleached corals were observed. Nevertheless, no differences were found in Symbiodinium community among time, environments, coral status and location, showing that O. patagonica hosted only one genotype of Symbiodinium belonging to clade B2. The fact that this clade has not been previously detected in other Mediterranean corals and is more frequent in the tropical Western Atlantic, is a new evidence that O. patagonica is an alien species in the Mediterranean Sea.
Patrocinador/es: We are grateful to the University of Alicante for awarding a predoctorate grant to ERP. This work was funded by projects CTM2012-38222-C02-02 (to AdlRM) and CGL2012-39627-C03-01 (to JA) of the Spanish Ministry of Economy and Competitiveness, which were also co-financed with FEDER support from the European Union (EU).
ISSN: 1874-7787 (Print) | 1876-7478 (Online)
DOI: 10.1016/j.margen.2014.06.002
Idioma: eng
Tipo: info:eu-repo/semantics/article
Derechos: © 2014 Elsevier B.V.
Revisión científica: si
Versión del editor:
Aparece en las colecciones:INV - BM - Artículos Científicos / Scientific Papers
INV - Recursos Hídricos y Desarrollo Sostenible - Artículos de Revistas
INV - EMM - Artículos de Revistas

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