RAPD and Phytochemical Analysis of Thymus moroderi Plantlets after Cryopreservation
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Título: | RAPD and Phytochemical Analysis of Thymus moroderi Plantlets after Cryopreservation |
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Autor/es: | Marco Medina, Ana | Casas, Jose L. |
Grupo/s de investigación o GITE: | Biodiversidad y Biotecnología aplicadas a la Biología de la Conservación |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Ciencias Ambientales y Recursos Naturales | Universidad de Alicante. Centro Iberoamericano de la Biodiversidad |
Palabras clave: | Cryopreservation | Essential oils | Molecular marker | RAPD | Thyme | Vitrification |
Área/s de conocimiento: | Fisiología Vegetal |
Fecha de publicación: | mar-2013 |
Editor: | CryoLetters |
Cita bibliográfica: | CryoLetters. 2013, 34(2): 119-127 |
Resumen: | Cryopreservation is at present the most reliable strategy to preserve plant germplasm. When aromatic plants are the object of conservation it is necessary to assess not only the genetic but also the phytochemical stability to ensure that plant material maintains its qualities after storage. In this work we present molecular and phytochemical stability data related to a previously described vitrification-based cryopreservation protocol for Thymus moroderi Pau ex Martínez. RAPD markers have been used to assess the genetic stability of T. moroderi explants and revealed 0.34% of variation in the cryopreserved material studied. Phytochemical data collected from GC-MS analysis of dichloromethane extracts from cryopreserved plantlets rendered a profile in which 1,8-cineole (14.5%), camphor (5.9%) and borneol (5.2%) were the major components. Both data confirmed the suitability of the cryopreservation protocol applied. |
Patrocinador/es: | A.M.-M. was financially supported by a Predoctoral fellowship (FPU) from the Spanish Ministry of Science and Innovation. |
URI: | http://hdl.handle.net/10045/42520 |
ISSN: | 0143-2044 (Print) | 1742-0644 (Online) |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Revisión científica: | si |
Versión del editor: | http://www.ingentaconnect.com/content/cryo/cryo/2013/00000034/00000002/art00002 |
Aparece en las colecciones: | INV - BBaBC - Artículos de Revistas |
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2013_Marco_Casas_CryoLetters.pdf | Acceso restringido | 2,69 MB | Adobe PDF | Abrir Solicitar una copia |
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