Early signaling events in grapevine cells elicited with cyclodextrins and methyl jasmonate
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Título: | Early signaling events in grapevine cells elicited with cyclodextrins and methyl jasmonate |
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Autor/es: | Belchí-Navarro, Sarai | Almagro, Lorena | Sabater Jara, Ana Belén | Fernández Pérez, Francisco | Bru-Martinez, Roque | Pedreño, María A. |
Grupo/s de investigación o GITE: | Proteómica y Genómica Funcional de Plantas |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Agroquímica y Bioquímica |
Palabras clave: | Cyclodextrins | Elicitation | Hydrogen peroxide | Methyl jasmonate | Nitric oxide | Trans-resveratrol | Vitis vinifera cell cultures |
Área/s de conocimiento: | Bioquímica y Biología Molecular |
Fecha de publicación: | ene-2013 |
Editor: | Elsevier |
Cita bibliográfica: | Plant Physiology and Biochemistry. 2013, 62: 107-110. doi:10.1016/j.plaphy.2012.11.001 |
Resumen: | The use of cyclic oligosaccharides like cyclodextrins (CDs), alone or combined with methyl jasmonate (MJ), as elicitors has proved very effective in stimulating the production of trans-resveratrol (trans-R) in Vitis vinifera suspension-cultured cells (SCC). Since elicitors can be used to increase trans-R production, understanding the molecular mechanisms involved would improve the management of grapevine cells as factories of this compound. The results obtained in this study provide evidence for a role of Ca2+ in mediating elicitor-induced trans-R production in grapevine SCC. The Ca2+ elevation was promoted by an uptake of Ca2+ from the extracellular medium, and by Ca2+ mobilization from intracellular organelles. Moreover, protein phosphorylation/dephosphorylation events seem to be involved in the signal transduction pathways triggered by CDs separately or in combination with MJ since trans-R production is dependent on both, the phosphorylation status of several proteins through mitogen-activated kinase pathway and the activity of tyrosine phosphatases. Our results also suggest that H2O2 and NO participated in the production of trans-R triggered by both elicitors in grapevine SCC. Finally, a fast alkalinization of the extracellular medium is induced in the presence of CDs and/or MJ. |
Patrocinador/es: | L. Almagro, AB. Sabater-Jara and F. Fernández-Pérez have grants from the MICINN. This study was supported by the Fundación Séneca, Agencia de Ciencia y Tecnología de la Región de Murcia en el marco de II PCTRM 2007e10 (08799/PI/08) and MICINN-FEDER (BIO2008-2941 and BIO2011-29856-C02-02). |
URI: | http://hdl.handle.net/10045/40207 |
ISSN: | 0981-9428 (Print) | 1873-2690 (Online) |
DOI: | 10.1016/j.plaphy.2012.11.001 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Revisión científica: | si |
Versión del editor: | http://dx.doi.org/10.1016/j.plaphy.2012.11.001 |
Aparece en las colecciones: | INV - Proteómica y Genómica Funcional de Plantas - Artículos de Revistas |
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