Electroretinographical and histological study of mouse retina after optic nerve section: a comparison between wild-type and retinal degeneration 1 mice

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Title: Electroretinographical and histological study of mouse retina after optic nerve section: a comparison between wild-type and retinal degeneration 1 mice
Authors: Germain Martínez, Francisco | Istillarte, Mirna | Gómez-Vicente, Violeta | Pérez Rico, Consuelo | Villa Polo, Pedro de la
Research Group/s: Neurobiología del Sistema Visual y Terapia de Enfermedades Neurodegenerativas (NEUROVIS)
Center, Department or Service: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología
Keywords: Electroretinogram | Optic neuropathy | Retina | Retinal degeneration
Knowledge Area: Biología Celular
Issue Date: Aug-2013
Publisher: Wiley
Citation: Clinical & Experimental Ophthalmology. 2013, 41(6): 593-602. doi:10.1111/ceo.12046
Abstract: Background: Retinal ganglion cell death underlies the pathophysiology of neurodegenerative disorders such as glaucoma or optic nerve trauma. To assess the potential influence of photoreceptor degeneration on retinal ganglion cell survival, and to evaluate functionality, we took advantage of the optic nerve section mouse model. Methods: Surviving retinal ganglion cells were double-stained by exposing both superior colliculi to fluorogold, and by applying dextran-tetramethylrhodamine to the injured optic nerve stump. To assess retinal function in wild-type animals, electroretinograms were recorded on the injured eyes and compared with the contralateral. Similar labelling experiments were carried out on retinal degeneration 1 mice. Surviving retinal ganglion cells were counted 21 days after axotomy and compared with wild-type mice. No functional experiments were performed on retinal degeneration 1 animals because they do not develop normal electroretinographical responses. Results: A significant decrease in retinal ganglion cell density was observed 6 days after axotomy in the wild type. Functional studies revealed that, in scotopic conditions, axotomy induced a significant amplitude decrease in the positive scotopic threshold response component of the electroretinogram. Such decrease paralleled cell loss, suggesting it may be an appropriate technique to evaluate functionality. When comparing retinal ganglion cell densities in wild-type and retinal degeneration 1 mice, a significant greater survival was observed on the latter. Conclusions: After optic nerve section, electroretinographical recordings exhibited a progressive decrease in the amplitude of the positive scotopic threshold response wave, reflecting ganglion cell loss. Interestingly, rod degeneration seemed, at least initially, to protect from axotomy-driven damage.
Sponsor: This research was supported by grants from the Spanish Ministerio de Educación y Ciencia (SAF2007-66175 and SAF2010-21879) and Instituto de Salud Carlos III (RD07/0062/0008) to PdlV.
URI: http://hdl.handle.net/10045/40110
ISSN: 1442-6404 (Print) | 1442-9071 (Online)
DOI: 10.1111/ceo.12046
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2012 The Authors. Clinical and Experimental Ophthalmology © 2012 Royal Australian and New Zealand College of Ophthalmologists. This is the pre-peer reviewed version of the following article: Clinical & Experimental Ophthalmology. 2013, 41(6): 593-602, which has been published in final form at http://dx.doi.org/10.1111/ceo.12046.
Peer Review: si
Publisher version: http://dx.doi.org/10.1111/ceo.12046
Appears in Collections:INV - NEUROVIS - Artículos de Revistas

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