Impairment of Intrinsically Photosensitive Retinal Ganglion Cells Associated With Late Stages of Retinal Degeneration
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http://hdl.handle.net/10045/40107
Títol: | Impairment of Intrinsically Photosensitive Retinal Ganglion Cells Associated With Late Stages of Retinal Degeneration |
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Autors: | Esquiva, Gema | Lax, Pedro | Cuenca, Nicolás |
Grups d'investigació o GITE: | Neurobiología del Sistema Visual y Terapia de Enfermedades Neurodegenerativas (NEUROVIS) |
Centre, Departament o Servei: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología |
Paraules clau: | Melanopsin | ipRGCs | Retinitis pigmentosa | P23H rats | Immunoperoxidase |
Àrees de coneixement: | Biología Celular | Fisiología |
Data de publicació: | 10-de juliol-2013 |
Editor: | Association for Research in Vision and Ophthalmology (ARVO) |
Citació bibliogràfica: | Investigative Ophthalmology & Visual Science. 2013, 54(7): 4605-4618. doi:10.1167/iovs.13-12120 |
Resum: | Purpose. To evaluate quantitative and qualitative age-related changes in intrinsically photosensitive melanopsin-containing retinal ganglion cells (ipRGCs) in transgenic P23H rats, an animal model of autosomal dominant retinitis pigmentosa (RP) was examined. Methods. ipRGC density, morphology, and integrity were characterized by immunohistochemistry in retinas extracted from P23H and Sprague–Dawley (SD) rats aged 4, 12, and 18 months. Differences between SD and P23H rats throughout the experimental stages, as well as the interactions among them, were morphologically evaluated. Results. In rat retinas, we have identified ipRGCs with dendrites stratifying in either the outer margin (M1) or inner side (M2) of the inner plexiform layer, and in both the outer and inner plexuses (M3). A small group of M1 cells had their somas located in the inner nuclear layer (M1d). In SD rats, ipRGCs showed no significant changes associated with age, in terms of either mean cell density or the morphologic parameters analyzed. However, the mean density of ipRGCs in P23H rats fell by approximately 67% between 4 and 18 months of age. Moreover, ipRGCs in these animals showed a progressive age-dependent decrease in the dendritic area, the number of branch points and terminal neurite tips per cell, and the Sholl area. Conclusions. In the P23H rat model of retinitis pigmentosa, density, wholeness, and dendritic arborization of melanopsin-containing ganglion cells decrease in advanced stages of the degenerative disease. |
Patrocinadors: | Supported by the Spanish Ministry of Economy and Competitiveness Grant BFU2012-36845; Instituto de Salud Carlos III Grant RETICS RD12/0034/0010; Universidad de Alicante Grant UA2010-48536273; and Organización Nacional de Ciegos Españoles (ONCE). |
URI: | http://hdl.handle.net/10045/40107 |
ISSN: | 0146-0404 (Print) | 1552-5783 (Online) |
DOI: | 10.1167/iovs.13-12120 |
Idioma: | eng |
Tipus: | info:eu-repo/semantics/article |
Drets: | © 2013 The Association for Research in Vision and Ophthalmology, Inc. |
Revisió científica: | si |
Versió de l'editor: | http://dx.doi.org/10.1167/iovs.13-12120 |
Apareix a la col·lecció: | INV - NEUROVIS - Artículos de Revistas |
Arxius per aquest ítem:
Arxiu | Descripció | Tamany | Format | |
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2013_Esquiva_etal_IOVS_final.pdf | Versión final (acceso restringido) | 1,63 MB | Adobe PDF | Obrir Sol·licitar una còpia |
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