Lidocaine effects on acetylcholine-elicited currents from mouse superior cervical ganglion neurons

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Título: Lidocaine effects on acetylcholine-elicited currents from mouse superior cervical ganglion neurons
Autor/es: Alberola-Die, Armando | Reboreda, Antonio | Lamas, J. Antonio | Morales, Andrés
Grupo/s de investigación o GITE: Fisiología de Membranas
Centro, Departamento o Servicio: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología
Palabras clave: Lidocaine | Local anaesthetics | Nicotinic receptors | Open-channel blockade | Closed-channel blockade | Allosteric modulation | Sympathetic neurons
Área/s de conocimiento: Fisiología
Fecha de publicación: mar-2013
Editor: Elsevier
Cita bibliográfica: Neuroscience Research. 2013, 75(3): 198-203. doi:10.1016/j.neures.2013.01.005
Resumen: Lidocaine is a commonly used local anaesthetic that, besides blocking voltage-dependent Na+ channels, has multiple inhibitory effects on muscle-type nicotinic acetylcholine (ACh) receptors (nAChRs). In the present study, we have investigated the effects of lidocaine on ACh-elicited currents (IAChs) from cultured mouse superior cervical ganglion (SCG) neurons, which mainly express heteromeric α3β4 nAChRs. Neurons were voltage-clamped by using the perforated-patch method and IAChs were elicited by fast application of ACh (100-300 μM), either alone or in presence of lidocaine at different concentrations. IAChs were reversibly blocked by lidocaine in a concentration-dependent way (IC50 = 41 μM; nH close to 1) and the inhibition was, at least partially, voltage-dependent, indicating an open-channel blockade. Besides, lidocaine blocked resting (closed) nAChRs, as evidenced by the increased inhibition caused by a 12 s lidocaine application just before its co-application with the agonist, and also enhanced IAChs desensitisation, at concentrations close to the IC50. These results indicate that lidocaine has diverse inhibitory actions on neuronal heteromeric nAChRs resembling those previously reported for Torpedo (muscle-type) nAChRs ( Alberola-Die et al., 2011). The similarity of lidocaine actions on different subtypes of heteromeric nAChRs differs with the specific effects of other compounds, restricted to particular subtypes of nAChRs.
Patrocinador/es: This work was supported by the following MICINN (Spanish government) grants: CONSOLIDER-INGENIO 2010 (CSD2008-00005), BFU2011-25371 and BFU2012-31359.
ISSN: 0168-0102 (Print) | 1872-8111 (Online)
DOI: 10.1016/j.neures.2013.01.005
Idioma: eng
Tipo: info:eu-repo/semantics/article
Revisión científica: si
Versión del editor:
Aparece en las colecciones:INV - Fisiología de Membranas - Artículos de Revistas

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