Retinal microglia are activated by systemic fungal infection

Please use this identifier to cite or link to this item:
Información del item - Informació de l'item - Item information
Title: Retinal microglia are activated by systemic fungal infection
Authors: Maneu, Victoria | Noailles, Agustina | Megías, Javier | Gómez-Vicente, Violeta | Carpena, Núria | Gil, María Luisa | Gozalbo, Daniel | Cuenca, Nicolás
Research Group/s: Neurobiología del Sistema Visual y Terapia de Enfermedades Neurodegenerativas (NEUROVIS)
Center, Department or Service: Universidad de Alicante. Departamento de Óptica, Farmacología y Anatomía | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología
Keywords: Microglial activation | Retina | Infection | Candidiasis
Knowledge Area: Farmacología | Biología Celular
Issue Date: 15-May-2014
Publisher: Association for Research in Vision and Ophthalmology (ARVO)
Citation: Investigative Ophthalmology & Visual Science. 2014, 55: 3578-3585. doi:10.1167/iovs.14-14051
Abstract: Purpose: To determine whether systemic fungal infection could cause activation of retinal microglia and therefore could be potentially harmful for patients with retinal degenerative diseases. Methods: Activation of retinal microglia was measured in a model of sublethal invasive candidiasis in C57BL/6J mice by (i) confocal immunofluorescence and (ii) flow cytometry analysis, using anti-CD11b, anti-Iba1, anti-MHCII and anti-CD45 antibodies. Results: Systemic fungal infection causes activation of retinal microglia, with phenotypic changes in morphology, surface markers expression, and microglial re-location in retinal layers. Conclusions: As an excessive or prolonged microglial activation may lead to chronic inflammation with severe pathological side effects, causing or worsening the course of retinal dystrophies, a systemic infection may represent a risk factor to be considered in patients with ocular neurodegenerative diseases, such as diabetic retinopathy, glaucoma, age-related macular degeneration or retinitis pigmentosa.
Sponsor: This work was supported by projects grants from the Spanish Ministry of Economy and Competitiveness-FEDER BFU2012-36845, Instituto de Salud Carlos III RETICS RD12/0034/0010 and ONCE to Nicolás Cuenca and Instituto de Salud Carlos III PI080556 to Daniel Gozalbo. Violeta Gómez-Vicente is a MICINN-Juan de la Cierva post-doctoral fellow (JCI-2009-05224).
ISSN: 0146-0404 (Print) | 1552-5783 (Online)
DOI: 10.1167/iovs.14-14051
Language: eng
Type: info:eu-repo/semantics/article
Rights: Copyright © 2014 by Association for Research in Vision and Ophthalmology
Peer Review: si
Publisher version:
Appears in Collections:INV - NEUROVIS - Artículos de Revistas

Files in This Item:
Files in This Item:
File Description SizeFormat 
Thumbnail2014_Maneu_etal_IOVS.pdfVersión revisada (acceso abierto)564,08 kBAdobe PDFOpen Preview
Thumbnail2014_Maneu_etal_IOVS_final.pdfVersión final (acceso restringido)743,11 kBAdobe PDFOpen    Request a copy

Items in RUA are protected by copyright, with all rights reserved, unless otherwise indicated.