CRISPR-Cas Functional Module Exchange in Escherichia coli

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dc.contributorMicrobiología Moleculares
dc.contributor.authorAlmendros, Cristóbal-
dc.contributor.authorMojica, Francisco J.M.-
dc.contributor.authorDíez-Villaseñor, César-
dc.contributor.authorGuzmán, Noemí M.-
dc.contributor.authorGarcía-Martínez, Jesús-
dc.contributor.otherUniversidad de Alicante. Departamento de Fisiología, Genética y Microbiologíaes
dc.identifier.citationAlmendros C, Mojica FJM, Díez-Villaseñor C, Guzmán NM, García-Martínez J. 2014. CRISPR-Cas functional module exchange in Escherichia coli. mBio 5(1):e00767-13. doi:10.1128/mBio.00767-13es
dc.description.abstractClustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (cas) genes constitute the CRISPR-Cas systems found in the Bacteria and Archaea domains. At least in some strains they provide an efficient barrier against transmissible genetic elements such as plasmids and viruses. Two CRISPR-Cas systems have been identified in Escherichia coli, pertaining to subtypes I-E (cas-E genes) and I-F (cas-F genes), respectively. In order to unveil the evolutionary dynamics of such systems, we analyzed the sequence variations in the CRISPR-Cas loci of a collection of 131 E. coli strains. Our results show that the strain grouping inferred from these CRISPR data slightly differs from the phylogeny of the species, suggesting the occurrence of recombinational events between CRISPR arrays. Moreover, we determined that the primary cas-E genes of E. coli were altogether replaced with a substantially different variant in a minor group of strains that include K-12. Insertion elements play an important role in this variability. This result underlines the interchange capacity of CRISPR-Cas constituents and hints that at least some functional aspects documented for the K-12 system may not apply to the vast majority of E. coli
dc.description.sponsorshipThis work was funded by the Ministerio de Economía y Competitividad (BIO2011-24417).es
dc.publisherAmerican Society for Microbiologyes
dc.rightsCopyright © 2014 Almendros et al. This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are
dc.subjectCRISPR-Cas systemses
dc.subjectEscherichia colies
dc.titleCRISPR-Cas Functional Module Exchange in Escherichia colies
Appears in Collections:INV - Microbiología Molecular - Artículos de Revistas

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