CRISPR-Cas Functional Module Exchange in Escherichia coli

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Títol: CRISPR-Cas Functional Module Exchange in Escherichia coli
Autors: Almendros, Cristóbal | Mojica, Francisco J.M. | Díez-Villaseñor, César | Guzmán, Noemí M. | García-Martínez, Jesús
Grups d'investigació o GITE: Microbiología Molecular
Centre, Departament o Servei: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología
Paraules clau: CRISPR-Cas systems | Escherichia coli
Àrees de coneixement: Microbiología
Data de publicació: 28-de gener-2014
Editor: American Society for Microbiology
Citació bibliogràfica: Almendros C, Mojica FJM, Díez-Villaseñor C, Guzmán NM, García-Martínez J. 2014. CRISPR-Cas functional module exchange in Escherichia coli. mBio 5(1):e00767-13. doi:10.1128/mBio.00767-13
Resum: Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (cas) genes constitute the CRISPR-Cas systems found in the Bacteria and Archaea domains. At least in some strains they provide an efficient barrier against transmissible genetic elements such as plasmids and viruses. Two CRISPR-Cas systems have been identified in Escherichia coli, pertaining to subtypes I-E (cas-E genes) and I-F (cas-F genes), respectively. In order to unveil the evolutionary dynamics of such systems, we analyzed the sequence variations in the CRISPR-Cas loci of a collection of 131 E. coli strains. Our results show that the strain grouping inferred from these CRISPR data slightly differs from the phylogeny of the species, suggesting the occurrence of recombinational events between CRISPR arrays. Moreover, we determined that the primary cas-E genes of E. coli were altogether replaced with a substantially different variant in a minor group of strains that include K-12. Insertion elements play an important role in this variability. This result underlines the interchange capacity of CRISPR-Cas constituents and hints that at least some functional aspects documented for the K-12 system may not apply to the vast majority of E. coli strains.
Patrocinadors: This work was funded by the Ministerio de Economía y Competitividad (BIO2011-24417).
URI: http://hdl.handle.net/10045/35463
ISSN: 2150-7511
DOI: 10.1128/mBio.00767-13
Idioma: eng
Tipus: info:eu-repo/semantics/article
Drets: Copyright © 2014 Almendros et al. This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
Revisió científica: si
Versió de l'editor: http://dx.doi.org/10.1128/mBio.00767-13
Apareix a la col·lecció: INV - Microbiología Molecular - Artículos de Revistas

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