High metabolomic microdiversity within co-occurring isolates of the extremely halophilic bacterium Salinibacter ruber

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Título: High metabolomic microdiversity within co-occurring isolates of the extremely halophilic bacterium Salinibacter ruber
Autor/es: Anton, Josefa | Lucio, Marianna | Peña Pardo, Aránzazu | Cifuentes, Ana | Brito-Echeverría, Jocelyn | Moritz, Franco | Tziotis, Dimitrios | López, Cristina | Urdiain, Mercedes | Schmitt-Kopplin, Philippe | Rosselló Móra, Ramon
Grupo/s de investigación o GITE: Ecología Microbiana Molecular
Centro, Departamento o Servicio: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología
Palabras clave: Salinibacter ruber | Halophilic bacterium | Isolates | Metabolomic microdiversity
Área/s de conocimiento: Microbiología
Fecha de publicación: 31-may-2013
Editor: Public Library of Science (PLoS)
Cita bibliográfica: Antón J, Lucio M, Peña A, Cifuentes A, Brito-Echeverría J, et al. (2013) High Metabolomic Microdiversity within Co-Occurring Isolates of the Extremely Halophilic Bacterium Salinibacter ruber. PLoS ONE 8(5): e64701. doi:10.1371/journal.pone.0064701
Resumen: Salinibacter ruber is an extremely halophilic member of the Bacteroidetes that thrives in crystallizer ponds worldwide. Here, we have analyzed two sets of 22 and 35 co-occurring S. ruber strains, newly isolated respectively, from 100 microliters water samples from crystalizer ponds in Santa Pola and Mallorca, located in coastal and inland Mediterranean Spain and 350 km apart from each other. A set of old strains isolated from the same setting were included in the analysis. Genomic and taxonomy relatedness of the strains were analyzed by means of PFGE and MALDI-TOF, respectively, while their metabolomic potential was explored with high resolution ion cyclotron resonance Fourier transform mass spectrometry (ICR-FT/MS). Overall our results show a phylogenetically very homogeneous species expressing a very diverse metabolomic pool. The combination of MALDI-TOF and PFGE provides, for the newly isolated strains, the same scenario presented by the previous studies of intra-specific diversity of S. ruber using a more restricted number of strains: the species seems to be very homogeneous at the ribosomal level while the genomic diversity encountered was rather high since no identical genome patterns could be retrieved from each of the samples. The high analytical mass resolution of ICR-FT/MS enabled the description of thousands of putative metabolites from which to date only few can be annotated in databases. Some metabolomic differences, mainly related to lipid metabolism and antibiotic-related compounds, provided enough specificity to delineate different clusters within the co-occurring strains. In addition, metabolomic differences were found between old and new strains isolated from the same ponds that could be related to extended exposure to laboratory conditions.
Patrocinador/es: This work was supported by the projects CLG2009-12651-C02-01 and 02; and CE-CSD2007-0005 of the Spanish Ministry of Science and Innovation, and all three projects were also co-financed with FEDER support from the European Union. JBE was financed by the Government of the Balearic Islands, Ministry of Economy and Finances.
URI: http://hdl.handle.net/10045/33641
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0064701
Idioma: eng
Tipo: info:eu-repo/semantics/article
Derechos: © 2013 Antón et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Revisión científica: si
Versión del editor: http://dx.doi.org/10.1371/journal.pone.0064701
Aparece en las colecciones:INV - EMM - Artículos de Revistas

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