Circadian dysfunction in P23H rhodopsin transgenic rats: effects of exogenous melatonin

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Título: Circadian dysfunction in P23H rhodopsin transgenic rats: effects of exogenous melatonin
Autor/es: Lax, Pedro | Baño Otalora, Beatriz | Esquiva Sobrino, Gema | Rol de Lama, María de los Ángeles | Madrid Pérez, Juan Antonio | Cuenca, Nicolás
Grupo/s de investigación o GITE: Neurobiología del Sistema Visual y Terapia de Enfermedades Neurodegenerativas (NEUROVIS)
Centro, Departamento o Servicio: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología
Palabras clave: Chronodisruption | Core temperature rhythm | Electroretinogram | Melatonin | P23H | Retinitis pigmentosa
Área/s de conocimiento: Oftalmología | Fisiología
Fecha de publicación: 9-nov-2010
Editor: John Wiley & Sons
Cita bibliográfica: LAX ZAPATA, Pedro, et al. “Circadian dysfunction in P23H rhodopsin transgenic rats: effects of exogenous melatonin”. Journal of Pineal Research. Vol. 50, Issue 2 (March 2011). ISSN 0742-3098, pp. 183-191
Resumen: This study focuses on the effects of retinal degeneration on the circadian patterns of P23H rats, as well as on the effect of exogenous melatonin administration. To this end, the body temperature of P23H and Sprague–Dawley rats was continuously monitored and their retinas examined at different stages of degeneration, by means of histological labeling and electroretinogram recordings. Melatonin (2 mg/kg BW/day) was supplied ad libitum throughout the experiment to a subset of animals. The body temperature recordings from wild-type and mutant animals showed no differences in the periodogram and the pattern of the mean waveform. However, a progressive decrease in the relative amplitude of the rhythm (RA), a decline in the coupling strength of the rhythm to environmental zeitgebers (interdaily stability, IS) and increased rhythm fragmentation (intradaily variability, IV) were observed in P23H rats, when compared to wild-type animals. The P23H animals showed a progressive decrease in light-induced retinal responses until reaching 18 months of age. By this age, all photoreceptors had already disappeared, and no responses were found in the EGRs. Exogenous administration of melatonin improved the visual response of P23H rats. In fact, the maximum b-wave recorded at 14 months of age was significantly higher in melatonin-treated P23H rats than in the control animals. Furthermore, the maximum b-wave recorded for P23H rats at the age of 14 months significantly correlated with RA, IS, and IV. This leads us to conclude that vision loss in P23H rats is correlated with a progressive fragmentation of their circadian patterns. Both effects are partially reversed by melatonin administration.
Patrocinador/es: This work was supported by MICINN (AP2006-04117, BFU2007-60658/BFI, and BFU2009-07793/BFI), Instituto de Salud Carlos III (RETICS RD07/0062/0012, RETICEF RD06/0013/0019), FUNDALUCE, ONCE, Fundación Médica Mutua Madrileña.
URI: http://hdl.handle.net/10045/16717
ISSN: 0742-3098 (Print) | 1600-079X (Online)
DOI: 10.1111/j.1600-079X.2010.00827.x
Idioma: eng
Tipo: info:eu-repo/semantics/article
Derechos: The definitive version is available at www3.interscience.wiley.com
Revisión científica: si
Versión del editor: http://dx.doi.org/10.1111/j.1600-079X.2010.00827.x
Aparece en las colecciones:INV - NEUROVIS - Artículos de Revistas

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