Environmental control of phosphorylation pathways in a branched two-component system

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Title: Environmental control of phosphorylation pathways in a branched two-component system
Authors: López Redondo, María Luisa | Moronta Barrios, Félix | Salinas, Paloma | Espinosa Manzano, Javier | Cantos, Raquel | Dixon, Ray | Marina Moreno, Alberto | Contreras, Asunción
Research Group/s: Transducción de Señales en Bacterias
Center, Department or Service: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología | Instituto de Biomedicina de Valencia | John Innes Centre. Department of Molecular Microbiology
Keywords: Two-component system | Cyanobacteria | Phosphorylation pathways
Knowledge Area: Genética
Date Created: 2010
Issue Date: 14-Sep-2010
Publisher: Blackwell Publishing
Citation: LÓPEZ REDONDO, María Luisa, et al. "Environmental control of phosphorylation pathways in a branched two-component system". Molecular Microbiology. Vol. 78, Issue 2 (Oct. 2010). ISSN 0950-382X, pp. 475-489
Abstract: NblS, the most conserved histidine kinase in cyanobacteria, regulates photosynthesis and acclimatization to a variety of environmental conditions. We used in silico, in vivo and in vitro approaches to identify RpaB and SrrA as the cognate response regulators of NblS and to characterize relevant interactions between components of this signalling system. While genetic analysis showed the importance of the NblS to RpaB phosphorylation branch for culture viability in Synechococcus elongatus PCC 7942, in vitro assays indicated a strong preference for NblS to phosphorylate SrrA. This apparent discrepancy can be explained by environmental insulation of the RpaB pathway, achieved by RpaB-dependent repression of srrA under standard, low light culture conditions. After a strong but transient increase in srrA expression upon high light exposure, negative regulation of srrA and other high light inducible genes takes place, suggesting cooperation between pathways under environmental conditions in which both RpaB and SrrA are present. Complex regulatory interactions between RpaB and SrrA, two response regulators with a common evolutionary origin that are controlled by a single histidine kinase, are thus emerging. Our results provide a paradigm for regulatory interactions between response regulators in a branched two-component system.
Sponsor: This work was supported by Ministerio de Ciencia e Innovación (Grants BFU2009-07371, BIO2009-10872 and Prometeo/2009/051) and Ministerio de Educación (PR2009-0378). Thanks to Sociedad de Relaciones Internacionales, Universidad de Alicante, for Felix Moronta's grant.
URI: http://hdl.handle.net/10045/14961
ISSN: 0950-382X (Print) | 1365-2958 (Online)
DOI: 10.1111/j.1365-2958.2010.07348.x
Language: eng
Type: info:eu-repo/semantics/article
Rights: The definitive version is available at www.blackwell-synergy.com
Peer Review: si
Publisher version: http://dx.doi.org/10.1111/j.1365-2958.2010.07348.x
Appears in Collections:INV - TSB - Artículos de Revistas

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