Phosphorylation-independent activation of the atypical response regulator NblR
Por favor, use este identificador para citar o enlazar este ítem:
http://hdl.handle.net/10045/14960
Título: | Phosphorylation-independent activation of the atypical response regulator NblR |
---|---|
Autor/es: | Ruiz Martinich, Diego | Salinas, Paloma | López Redondo, María Luisa | Cayuela Fuentes, María Luisa | Marina Moreno, Alberto | Contreras, Asunción |
Grupo/s de investigación o GITE: | Transducción de Señales en Bacterias |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología | Instituto de Biomedicina de Valencia |
Palabras clave: | NblR | Response regulator | PIARR |
Área/s de conocimiento: | Genética |
Fecha de creación: | 2008 |
Fecha de publicación: | 2008 |
Editor: | Society for General Microbiology |
Cita bibliográfica: | RUIZ MARTINICH, Diego, et al. “Phosphorylation-independent activation of the atypical response regulator NblR”. Microbiology. 154 (2008). ISSN 1350-0872, pp. 3002-3015 |
Resumen: | Cyanobacteria respond to environmental stress conditions by adjusting their photosynthesis machinery. In Synechococcus sp. PCC 7942, phycobilisome degradation and other acclimation responses after nutrient or high-light stress require activation by the orphan response regulator NblR, a member of the OmpR/PhoB family. Although NblR contains a putative phosphorylatable residue (Asp57), it lacks other conserved residues required to chelate the Mg2+ necessary for aspartic acid phosphorylation or to transduce the phosphorylation signal. In close agreement with these features, NblR was not phosphorylated in vitro by the low-molecular-mass phosphate donor acetyl phosphate and mutation of Asp57 to Ala had no impact on previously characterized NblR functions in Synechococcus. On the other hand, in vitro and in vivo assays show that the default state of NblR is monomeric, suggesting that, despite input differences, NblR activation could involve the same general mechanism of activation by dimerization present in known members of the OmpR/PhoB family. Structural and functional data indicate that the receiver domain of NblR shares similarities with other phosphorylation-independent response regulators such as FrzS and HP1043. To acknowledge the peculiarities of these atypical ‘two-component’ regulators with phosphorylation-independent signal transduction mechanisms, we propose the term PIARR, standing for phosphorylation-independent activation of response regulator. |
Patrocinador/es: | This work was supported by the Ministerio de Educación y Ciencia (grants BFU2006-12424 to A.C. and BIO2005-00153 to A. M.) and the Generalitat Valenciana (grant ACOMP06/083 to A.C.). |
URI: | http://hdl.handle.net/10045/14960 |
ISSN: | 1350-0872 (Print) | 1465-2080 (Online) |
DOI: | 10.1099/mic.0.2008/020677-0 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | This is an author manuscript that has been accepted for publication in Microbiology, copyright Society for General Microbiology, but has not been copy-edited, formatted or proofed. Cite this article as appearing in Microbiology. This version of the manuscript may not be duplicated or reproduced, other than for personal use or within the rule of 'Fair Use of Copyrighted Materials' (section 17, Title 17, US Code), without permission from the copyright owner, Society for General Microbiology. The Society for General Microbiology disclaims any responsibility or liability for errors or omissions in this version of the manuscript or in any version derived from it by any other parties. The final copy-edited, published article, which is the version of record, can be found at http://mic.sgmjournals.org, and is freely available without a subscription. |
Revisión científica: | si |
Aparece en las colecciones: | INV - TSB - Artículos de Revistas |
Archivos en este ítem:
Archivo | Descripción | Tamaño | Formato | |
---|---|---|---|---|
RuizMicrobiol2008.pdf | Articulo principal. Versión final (acceso restringido) | 471,52 kB | Adobe PDF | Abrir Solicitar una copia |
SupplementaryFig1.pdf | Supplementary Figure1 | 79,25 kB | Adobe PDF | Abrir Vista previa |
RuizetalJunio08Rev2.pdf | Preprint (acceso libre) | 241,28 kB | Adobe PDF | Abrir Vista previa |
Todos los documentos en RUA están protegidos por derechos de autor. Algunos derechos reservados.