Benchmarking of single‐virus genomics: a new tool for uncovering the virosphere
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Título: | Benchmarking of single‐virus genomics: a new tool for uncovering the virosphere |
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Autor/es: | Garcia-Heredia, Inmaculada | Bhattacharjee, Ananda S. | Fornas, Oscar | Lluesma Gómez, Mónica | Martínez Martínez, Joaquín | Martinez-Garcia, Manuel |
Grupo/s de investigación o GITE: | Ecología Microbiana Molecular |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología |
Palabras clave: | Single‐virus genomics | Viral single-amplified genomes | Whole-genome amplification | Viral ecology | Genome sequencing |
Área/s de conocimiento: | Microbiología |
Fecha de publicación: | mar-2021 |
Editor: | Society for Applied Microbiology | John Wiley & Sons |
Cita bibliográfica: | Environmental Microbiology. 2021, 23(3): 1584-1593. https://doi.org/10.1111/1462-2920.15375 |
Resumen: | Metagenomics and single‐cell genomics have enabled the discovery of relevant uncultured microbes. Recently, single‐virus genomics (SVG), although still in an incipient stage, has opened new avenues in viral ecology by allowing the sequencing of one single virus at a time. The investigation of methodological alternatives and optimization of existing procedures for SVG is paramount to deliver high‐quality genomic data. We report a sequencing dataset of viral single‐amplified genomes (vSAGs) from cultured and uncultured viruses obtained by applying different conditions in each SVG step, from viral preservation and novel whole‐genome amplification (WGA) to sequencing platforms and genome assembly. Sequencing data showed that cryopreservation and mild fixation were compatible with WGA, although fresh samples delivered better genome quality data. The novel TruPrime WGA, based on primase‐polymerase features, and WGA‐X employing a thermostable phi29 polymerase, were proven to be with sufficient sensitivity in SVG. The Oxford Nanopore (ON) sequencing platform did not provide a significant improvement of vSAG assembly compared to Illumina alone. Finally, the SPAdes assembler performed the best. Overall, our results represent a valuable genomic dataset that will help to standardized and advance new tools in viral ecology. |
Patrocinador/es: | This work has been supported by Gordon and Betty Moore Foundation (grant 5334) and Spanish Ministry of Economy and Competitiveness (refs CGL2013‐40564‐R, RTI2018‐094248‐B‐I00 and SAF2013‐49267‐EXP). Work at CRG, BIST and UPF was in part funded by the Spanish Ministry of Economy and Competitiveness, ‘Centro de Excelencia Severo Ochoa 2013‐2017’ and the Spanish Ministry of Economy and Competitiveness, ‘Centro de Excelencia Maria de Maeztu 2016‐2019’. |
URI: | http://hdl.handle.net/10045/113907 |
ISSN: | 1462-2912 (Print) | 1462-2920 (Online) |
DOI: | 10.1111/1462-2920.15375 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2020 Society for Applied Microbiology and John Wiley & Sons Ltd. |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.1111/1462-2920.15375 |
Aparece en las colecciones: | INV - EMM - Artículos de Revistas |
Archivos en este ítem:
Archivo | Descripción | Tamaño | Formato | |
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Garcia-Heredia_etal_2021_EnvironMicrobiol_final.pdf | Versión final (acceso restringido) | 565,28 kB | Adobe PDF | Abrir Solicitar una copia |
Garcia-Heredia_etal_2021_EnvironMicrobiol_accepted.pdf | Accepted Manuscript (acceso abierto) | 1,83 MB | Adobe PDF | Abrir Vista previa |
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