Chitosan modulates Pochonia chlamydosporia gene expression during nematode egg parasitism
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Título: | Chitosan modulates Pochonia chlamydosporia gene expression during nematode egg parasitism |
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Autor/es: | Suarez-Fernandez, Marta | Sambles, Christine | Lopez-Moya, Federico | Nueda, María José | Studholme, David J. | Lopez-Llorca, Luis Vicente |
Grupo/s de investigación o GITE: | Fitopatología | Bioquímica Aplicada/Applied Biochemistry (AppBiochem) | Grupo de Estadística Aplicada (GESTA) |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Ciencias del Mar y Biología Aplicada | Universidad de Alicante. Departamento de Matemáticas |
Palabras clave: | Plant‐parasitic nematodes | Pochonia chlamydosporia | Gene expression | Nematode egg parasitism | Chitosan |
Área/s de conocimiento: | Botánica | Estadística e Investigación Operativa |
Fecha de publicación: | 26-ene-2021 |
Editor: | Society for Applied Microbiology | John Wiley & Sons |
Cita bibliográfica: | Environmental Microbiology. 2021, 23(9): 4980-4997. https://doi.org/10.1111/1462-2920.15408 |
Resumen: | Climate change makes plant‐parasitic nematodes (PPN) an increasing threat to commercial crops. PPN can be managed sustainably by the biocontrol fungus Pochonia chlamydosporia (Pc). Chitosan generated from chitin deacetylation enhances PPN parasitism by Pc. In this work, we investigate the molecular mechanisms of Pc for chitosan resistance and root‐knot nematode (RKN) parasitism, using transcriptomics. Chitosan and RKN modify the expression of Pc genes, mainly those involved in oxidation–reduction processes. Both agents significantly modify the expression of genes associated to 113 GO terms and 180 Pc genes. Genes encoding putative glycoproteins (Pc adhesives) to nematode eggshell, as well as genes involved in redox, carbohydrate and lipid metabolism trigger the response to chitosan. We identify genes expressed in both the parasitic and endophytic phases of the Pc lifecycle; these include proteases, chitosanases and transcription factors. Using the Pathogen—Host Interaction database (PHI‐base), our previous RNA‐seq data and RT‐PCR of Pc colonizing banana we have investigated genes expressed both in the parasitic and endophytic phases of Pc lifecycle. |
Patrocinador/es: | This work was supported by H2020 MUSA 727624 European Project. |
URI: | http://hdl.handle.net/10045/113834 |
ISSN: | 1462-2912 (Print) | 1462-2920 (Online) |
DOI: | 10.1111/1462-2920.15408 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2021 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.1111/1462-2920.15408 |
Aparece en las colecciones: | INV - Fitopatología - Artículos de Revistas INV - GESTA - Artículos de Revistas Investigaciones financiadas por la UE INV - AppBiochem - Artículos de Revistas |
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